A novel fluorescently labelled ligand for the detection of dat in immune cells by flow cytometry

Access through your institution Buy or subscribe The role of dopamine, its activation of dopamine receptors, and reuptake at the dopamine transporter (DAT) has been primarily studied in the

central nervous system (CNS). However, dopamine signaling also extends to the periphery affecting immunity [1]. The DAT is the primary regulator of dopamine signaling and has been implicated

as an important player in the regulation of immune response. However, to what extent DAT expression and function affects immunity, the mechanisms underlying the bidirectional communication

of peripheral immune cells within the CNS, and how DAT is affected in disease states remain unclear [1]. Developing selective tools that enable visualization of DAT on immune cells can

provide new opportunities to advance our understanding of its role in immunity and as a potential peripheral biomarker. Fluorescently labelled ligands (FLL) are resourceful small molecules

that have facilitated investigation of the expression, distribution and internalization mechanisms of biologically relevant proteins. For many years, our efforts have been centered on

providing the scientific community with an extensive variety of FLL to study monoamine transporters (MATs) [2]. However, the lack of a DAT selective tool limited the exploration in cellular

systems where DAT and either the serotonin or the norepinephrine transporter (SERT and NET, respectively) were co-expressed. This is a preview of subscription content, access via your

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* Learn about institutional subscriptions * Read our FAQs * Contact customer support CHANGE HISTORY * _ 19 MARCH 2025 A Correction to this paper has been published:

https://doi.org/10.1038/s41386-025-02087-2 _ REFERENCES * Channer B, Matt SM, Nickoloff-Bybel EA, Pappa V, Agarwal Y, Wickman J, et al. Dopamine, immunity, and disease. Pharm Rev.

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Scholar  Download references FUNDING Funding Funding for this work was provided by the National Institute on Drug Abuse-Intramural Research Program (Z1A DA000389 to AHN). AUTHOR INFORMATION

AUTHORS AND AFFILIATIONS * Medicinal Chemistry Section, Molecular Targets and Medications Discovery Branch, National Institute on Drug Abuse – Intramural Research Program, National

Institutes of Health, Baltimore, MD, 21224, USA Gisela Andrea Camacho-Hernandez & Amy Hauck Newman Authors * Gisela Andrea Camacho-Hernandez View author publications You can also search

for this author inPubMed Google Scholar * Amy Hauck Newman View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS GACH wrote the first draft, AHN

edited, both authors approved the final version of manuscript. CORRESPONDING AUTHORS Correspondence to Gisela Andrea Camacho-Hernandez or Amy Hauck Newman. ETHICS DECLARATIONS COMPETING

INTERESTS The authors declare no competing interests. ADDITIONAL INFORMATION PUBLISHER’S NOTE Springer Nature remains neutral with regard to jurisdictional claims in published maps and

institutional affiliations. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Camacho-Hernandez, G.A., Newman, A.H. A novel fluorescently labelled ligand

for the detection of DAT in immune cells by flow cytometry. _Neuropsychopharmacol._ 50, 349–350 (2025). https://doi.org/10.1038/s41386-024-01935-x Download citation * Published: 24 July 2024

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