1557 an explanation for the effect of reye's syndrome serum on preparations of isolated mitochondria


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ABSTRACT Reye's Syndrome (RS) serum added to isolated rat liver mitochondria caused a stimulation of O2 consumption [BBRC 79:1122 (1977)]. This suggested a metabolic toxin in the serum


with activity against mitochondria. Recently, the substance responsible for the serum activity was identified as uric acid [BBRC 94:381 (1980)]. However, it appeared that uric acid had no


direct effect on mitochondria. Instead, it was suspected that the stimulation of O2 consumption was a result of the oxidation of the added uric acid by peroxisomes, which are usually present


with rat liver mitochondria prepared by differential centrifugation. This was confirmed by separating mitochondria and peroxisomes, in which case the serum effect followed the peroxisomal


fraction. Further study showed that all of the serum activity detected in the bioassay was attributable to uric acid oxidation. The concentration of uric acid in serum was directly


correlated with the magnitude of the serum effect on O2 utilization, and uricase treatment of serum abolished its effect on O2 consumption. In summary, the particular bioassay used in these


studies detected uric acid, which is not a genuine pathogenic factor in RS. The search for “serum factors” should be continued using this general approach, but employing different bioassays


to detect adverse effects on metabolic function. (Supp. by NIH NS 14936) ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department Biology, Tufts University, Medford, MA. June R


Aprille Authors * June R Aprille View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE


THIS ARTICLE Aprille, J. 1557 AN EXPLANATION FOR THE EFFECT OF REYE'S SYNDROME SERUM ON PREPARATIONS OF ISOLATED MITOCHONDRIA. _Pediatr Res_ 15 (Suppl 4), 702 (1981).


https://doi.org/10.1203/00006450-198104001-01574 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01574 SHARE THIS ARTICLE Anyone you share the


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