Tuning expression by numbers | Nature Methods


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You have full access to this article via your institution. Download PDF Brewster, R.C. _et al_. _Cell_ 156, 1312–1323 (2014). When predicting the regulatory effect of a transcription factor


(TF) in bacterial gene expression, many models assume the TF to be in excess of its binding sites on a promoter. But as Brewster _et al_. recently pointed out, this assumption is not always


valid, particularly in cases where genes are expressed at high copy numbers or where many genes compete for a TF. The researchers describe a simple regulatory circuit in which a TF represses


a reporter to measure the relationship between TF and various reporter copy numbers in the absence or presence of competing TF-binding sites. The resulting model will help fine-tune the


output of a genetic circuit without requiring modification of the regulated gene. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tuning expression by


numbers. _Nat Methods_ 11, 475 (2014). https://doi.org/10.1038/nmeth.2942 Download citation * Published: 29 April 2014 * Issue Date: May 2014 * DOI: https://doi.org/10.1038/nmeth.2942 SHARE


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