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ABSTRACT Blunt end ligation with an _Eco_RI/ octanucleotide “linker” was used to construct a plasmid containing chemically synthesized lac operator DNA. This plasmid renders the host
bacterium constitutive for β-galactosidase. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access
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Article 23 November 2020 SINGLE 3′-EXONUCLEASE-BASED MULTIFRAGMENT DNA ASSEMBLY METHOD (SENAX) Article Open access 07 March 2022 BACPE: A VERSATILE PRIME-EDITING PLATFORM IN BACTERIA BY
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references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * University of California, San Francisco, California, 94143 Herbert L. Heyneker, John Shine, Howard M. Goodman & Herbert W. Boyer
* California Institute of Technology, Pasadena, California, 91106 John Rosenberg & Richard E. Dickerson * National Research Council of Canada, Ottawa, Ontario, K1A OR6, Canada Saran A.
Narang * City of Hope National Medical Center, Duarte, California, 91010 Keiichi Itakura, Syr-yaung Lin & Arthur D. Riggs Authors * Herbert L. Heyneker View author publications You can
also search for this author inPubMed Google Scholar * John Shine View author publications You can also search for this author inPubMed Google Scholar * Howard M. Goodman View author
publications You can also search for this author inPubMed Google Scholar * Herbert W. Boyer View author publications You can also search for this author inPubMed Google Scholar * John
Rosenberg View author publications You can also search for this author inPubMed Google Scholar * Richard E. Dickerson View author publications You can also search for this author inPubMed
Google Scholar * Saran A. Narang View author publications You can also search for this author inPubMed Google Scholar * Keiichi Itakura View author publications You can also search for this
author inPubMed Google Scholar * Syr-yaung Lin View author publications You can also search for this author inPubMed Google Scholar * Arthur D. Riggs View author publications You can also
search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Heyneker, H., Shine, J., Goodman, H. _et al._ Synthetic
_lac_operator DNA is functional _in vivo_. _Nature_ 263, 748–752 (1976). https://doi.org/10.1038/263748a0 Download citation * Received: 29 July 1976 * Accepted: 30 August 1976 * Issue Date:
28 October 1976 * DOI: https://doi.org/10.1038/263748a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable
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