Increased expression and no mutation of the flap endonuclease (fen1) gene in human lung cancer

ABSTRACT The underlying molecular mechanisms leading to microsatellite alteration and mutations in human lung cancer remain unknown. Since _Flap endonuclease1_ (_Fen1_), which functions in

the base excision repair system, has been shown to be involved in tumor progression of mouse models with microsatellite instability in a haplo-insufficient manner, we performed expression

and mutation analyses for _FEN1_ in human lung cancer cell lines. Reverse transcriptase PCR analysis revealed that all 49 lung cancer cell lines (20 small cell lung cancers (SCLCs) and 29

non-small cell lung cancers (NSCLCs)) expressed _FEN1_. In addition, microarray analysis showed that _FEN1_ expression was elevated significantly by 1.65-fold (_P_=0.001) in SCLC cell lines

compared to normal lung controls (normal human lung cultures and immortalized normal human bronchial epithelial cell lines). FEN1 protein was abundantly expressed in all 23 lung cancer cell

lines (10 SCLCs and 13 NSCLCs) and was expressed at lower levels in three of four normal lung epithelial culture controls. Direct sequencing of genomic DNAs revealed no _FEN1_ mutation in

seven SCLCs and nine NSCLCs. As part of this analysis we discovered and sequenced a _FEN1_ pseudogene (GenBank accession #AY249897) located at 1p22.2. This pseudogene is amplified from cDNA

preparations contaminated with genomic DNA and must be taken into account in any _FEN1_ mutation analysis studies. Our results suggest that alterations of _FEN1_ are not likely to contribute

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FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS WHOLE‑EXOME SEQUENCING REVEALS LEWIS LUNG CARCINOMA IS A HYPERMUTATED _KRAS_/_NRAS_–MUTANT CANCER WITH EXTENSIVE

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01 February 2022 INTEGRATIVE GENOMICS IDENTIFIES SHPRH AS A TUMOR SUPPRESSOR GENE IN LUNG ADENOCARCINOMA THAT REGULATES DNA DAMAGE RESPONSE Article Open access 18 June 2024 ABBREVIATIONS *

SCLC: small cell lung cancer * NSCLC: non-small cell lung cancer * HNPCC: hereditary nonpolyposis colon cancer * RT–PCR: reverse transcriptase PCR * MSI: microsatellite instability * MMR,

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P50CA70907, and the Gillson Longenbaugh Foundation. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Internal Medicine, Hamon Center for Therapeutic Oncology Research, University

of Texas Southwestern Medical Center, Dallas, 75390-8593, TX, USA Mitsuo Sato, Luc Girard, Ikuo Sekine, Noriaki Sunaga, Ruben D Ramirez, Craig Kamibayashi & John D Minna * Department of

Pharmacology, Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, 75390-8593, TX, USA Mitsuo Sato, Luc Girard, Ikuo Sekine, Noriaki

Sunaga, Ruben D Ramirez, Craig Kamibayashi & John D Minna Authors * Mitsuo Sato View author publications You can also search for this author inPubMed Google Scholar * Luc Girard View

author publications You can also search for this author inPubMed Google Scholar * Ikuo Sekine View author publications You can also search for this author inPubMed Google Scholar * Noriaki

Sunaga View author publications You can also search for this author inPubMed Google Scholar * Ruben D Ramirez View author publications You can also search for this author inPubMed Google

Scholar * Craig Kamibayashi View author publications You can also search for this author inPubMed Google Scholar * John D Minna View author publications You can also search for this author

inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to John D Minna. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sato, M., Girard, L.,

Sekine, I. _et al._ Increased expression and no mutation of the _Flap endonuclease_ (_FEN1_) gene in human lung cancer. _Oncogene_ 22, 7243–7246 (2003).

https://doi.org/10.1038/sj.onc.1206977 Download citation * Received: 24 March 2003 * Revised: 25 June 2003 * Accepted: 26 June 2003 * Published: 16 October 2003 * Issue Date: 16 October 2003

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not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * _Flap endonuclease 1_ (_FEN1_) * base excision

repair * microsatellite instability * lung cancer